What is a FAM dye?

Fluorescein amidites, abbreviated as FAM, are important synthetic equivalents of fluorescein dye used in oligonucleotide synthesis and molecular biology. Oligonucleotides labeled with fluorescein at one of the termini and with a quencher at the other can serve as molecular beacons.

What is FAM in chemistry?

A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein.

What is FAM qPCR?

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5′ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3′ end.

Is fluorescent dye toxic?

Fluorescein is a widely used, FDA-approved fluorescent dye. Based on the existing data, it can have toxic effects in cell culture and on patients at high doses.

What is Rhodamine B dye?

Rhodamine B /ˈroʊdəmiːn/ is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with fluorometers.

What is FAM TaqMan?

What is 6-FAM dye used for?

6-Carboxyfluorescein ( 6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents.

Is 6-FAM a fluorescein?

?) 6-Carboxyfluorescein ( 6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added.

What is 6-FAM used for in PCR?

6-carboxyfluorescein (6-FAM), representing the most commonly used fluorescent dye for labeling oligonucleotides, is reactive and water-soluble (absorbance maximum of 492 nm; emission maximum of 517 nm). 6-FAM especially useful in real-time PCR, being used as a reporter moiety in TaqMan probes, Scorpion primers, and Molecular Beacons (1,2,3).

What is 6-carboxyfluorescein?

6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents. It is used in the sequencing of nucleic acids and in the labeling of nucleotides.