What does EGTA stand for?

EGTA (ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid), also known as egtazic acid (INN, USAN), is an aminopolycarboxylic acid, a chelating agent.

What is the purpose of EGTA in this experiment?

Ethylene glycol tetraacetic acid (EGTA) is also a chelating agent. Compared to EDTA, it has a higher affinity for calcium ions but a lower affinity for magnesium ions. Similar to EDTA, EGTA can be used as a buffer to resemble the pH of a living cell.

Does EGTA bind zinc?

Thus, EGTA is a much more robust competitor for proteomic Zn2+ than apo-MT yet binds Zn2+ with an apparent stability constant 2 orders of magnitude less than apo-MT.

Can I replace EGTA with EDTA?

As mentioned earlier, the two acids can be utilized as a chelating agent. Nonetheless, EDTA and EGTA don’t bind the same way. EGTA can be more suitable to be used with a divalent calcium cation. EDTA, on the other hand, is observed to be more attracted to a divalent magnesium cation.

How does EGTA chelate calcium?

EGTA is a chelating agent that is related to the better known EDTA, but with a much higher affinity for Ca2+than for Ca2+ ions. The pKa for binding of calcium ions by tetrabasic EGTA is 11.00, but the protonated forms do not significantly contribute to binding, so at pH 7, the apparent pKa becomes 6.91.

How do you dissolve EGTA in water?

For a 100 mM EGTA stock solution, add 3.8 g to about 20 ml of distilled H2O and bring to pH 11 with NaOH; then bring to pH 8.0 with HCl and add H2O to a final volume of 100 ml. Dissolve and adjust to 150 ml with ultrapure H2O. Filter-sterilize and store at room temperature.

What metals does EGTA chelate?

EGTA is useful for making buffer solutions to chelate calcium ions when calcium ions are less concentrated than magnesium, as found inside living cells – usually at least a thousand fold less concentrated.

How long is EGTA stable?

Store at 4°C (will last ~1 month). Use 1:100 in liq- uid and solid media (final 10 mM). For a 100 mM EGTA stock solution, add 3.8 g to about 20 ml of distilled H2O and bring to pH 11 with NaOH; then bring to pH 8.0 with HCl and add H2O to a final volume of 100 ml.

Is EGTA water soluble?

Solubility : Soluble in 1M NaOH (100 mM), Slightly soluble in water.

Does EGTA dissolve in water?

Use NaOH to dissolve EGTA and then make up volumnwith water you needed. make concentrated stock and dilute in PBS. Note: EGTA will not go into solution without NaOH. Once the pH has been raised sufficiently it dissolves quickly.

Is EGTA a buffer?

Ethylene glycol tetraacetic acid (EGTA) is a common buffer ingredient due to its chelating activity. Buffers made with EGTA are used in some cases to mimic the environment inside living cells where calcium ions are usually at least a thousandfold less concentrated than magnesium ions.

How do you store EGTA?

What is egegta?

EGTA ( ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid ), also known as egtazic acid ( INN, USAN ), is an aminopolycarboxylic acid, a chelating agent. It is a colourless solid that is related to the better known EDTA. Compared to EDTA, it has a lower affinity for magnesium, making it more selective for calcium ions.

What is the difference between EGTA and EDTA?

EGTA (chemical) EGTA ( ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid ), also known as egtazic acid ( INN, USAN ), is an aminopolycarboxylic acid, a chelating agent. It is a colourless solid that is related to the better known EDTA. Compared to EDTA, it has a lower affinity for magnesium,…

What is the chemical name of EGTA?

EGTA (chemical) From Wikipedia, the free encyclopedia EGTA (ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid), also known as egtazic acid (INN, USAN), is an aminopolycarboxylic acid, a chelating agent. It is a white solid that is related to the better known EDTA.