Is Bafilomycin reversible?

Is Bafilomycin reversible?

Bafilomycin A1 is a selective and reversible inhibitor of vacuolar H+ ATPases (V-ATPases) with IC50 value of 4-400 nM.

How do you dissolve Bafilomycin?

Bafilomycin A1 is supplied as a crystalline solid. A stock solution may be made by dissolving the bafilomycin A1 in the solvent of choice, which should be purged with an inert gas. Bafilomycin A1 is soluble in organic solvents such as DMSO and methanol.

What is an autophagy inhibitor?

Autophagy inhibitors, such as chloroquine and hydroxychloroquine, have already been clinically approved, promoting drug combination treatment by targeting autophagic pathways as a means of discovering and developing more novel and more effective cancer therapeutic approaches.

What does Bafilomycin A1 do?

Bafilomycin A1 is an inhibitor of vacuolar H+-ATPase that is frequently used at high concentration to block late-phase autophagy. Here, we show that bafilomycin A1 at a low concentration (1 nM) effectively and specifically inhibited and killed pediatric B-cell acute lymphoblastic leukemia cells.

How does Bafilomycin A1 inhibit autophagy?

Bafilomycin A1 (BafA1), a macrolide antibiotic, is a known inhibitor of the latter stages of autophagy, inhibiting fusion between autophagosomes and lysosomes by inhibiting vacuolar H+ ATPase (10).

How does hydroxychloroquine inhibit autophagy?

CQ and HCQ can inhibit autophagy by blocking the fusion of autophagosomes with lysosomes. In addition, CQ also has some autophagy-independent anticancer effects, including sensitizing cancer cells to chemotherapy. Several related clinical trials have been conducted to evaluate the safety and value of it.

What is autophagy induction?

Autophagy is induced in response to starvation, oxidative stress and accumulation of misfolded proteins12,13,14 and can serve to provide novel building blocks in response to high energy demands or as a protein quality control mechanism to remove protein aggregates or damaged organelles15.

What is the product usage information for Bafilomycin A1?

Product Usage Information. Bafilomycin A1 is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 100 μg in 160.56 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10 nM-1 μM for up to 18 hr.

Is s1413 Bafilomycin A1 soluble in DMSO?

S1413 Bafilomycin A1 (Baf-A1) is soluble in DMSO at 6 mg/ml. Please do not use alcohols as solvent, because this compound will degrade in alcohols.

What is the mechanism of action of Bafilomycin A1 in Helicobacter pylori infection?

Bafilomycin A1 prevents the vacuolization of Hela cells induced by H. pylori, with an inhibitory concentration giving 50% of maximal (ID50) of 4 nM. Bafilomycin A1 is also very efficient in restoring vacuolated cells to a normal appearance. [4]

What are the citations for Bafilomycin A1 (TGF-β-induced DACT1)?

Selected citations for Bafilomycin A1 include: Esposito et al (2021) TGF-β-induced DACT1 biomolecular condensates repress Wnt signalling to promote bone metastasis. Nat.Cell.Biol. 23 257 PMID: 33723425