Do phages have DNA polymerase?
Family A DNA polymerase is the primary enzyme responsible for phage genome replication (Doublie et al., 1998) within the ca. 25% of all known double-stranded DNA (dsDNA) phage known to carry this gene (Wommack et al., 2015).
Which DNA polymerase is found in E coli?
DNA Polymerase I, encoded by the polA gene, the first DNA polymerase discovered, is the most abundant polymerase in E. coli (approximately 400 molecules per cell) (Kornberg and Baker, 1992).
Which DNA polymerase in Escherichia coli has 5 to 3 exonuclease activity?
DNA polymerase III
DNA polymerase III (polIII) holoenzyme of Escherichia coli has 3′—-5′ exonuclease (“editing”) activity in addition to its polymerase activity, a property shared by other prokaryotic DNA polymerases. The polymerization activity is carried by the large alpha subunit, the product of the dnaE gene.
Do bacteriophages have double stranded DNA?
Bacteriophages are double-stranded DNA viruses that infect bacteria, and bacteriophage T4 infects Escherichia coli. Infection is usually followed by incorporation of phage DNA into the host cell genome as a prophage (lysogeny).
Do bacteriophages have DNA?
Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome, and may have structures that are either simple or elaborate. Their genomes may encode as few as four genes (e.g. MS2) and as many as hundreds of genes.
What is strand displacement?
The term strand displacement describes the ability to displace downstream DNA encountered during synthesis. NEB produces DNA polymerases with varying degrees of strand displacement activity. These polymerases are active at moderate temperatures, around 20–37°C.
How many polymerases are in E coli?
five DNA polymerases
There are five DNA polymerases in Escherichia coli (reviewed [1]). The main replicative polymerase is Pol III.
Which E coli DNA polymerase has the highest Processivity and fidelity?
DNA Pol III HE
The major replicative DNA polymerase in E. coli cells is DNA Pol III HE that can synthesize DNA with high processivity (over 50 kb per binding event) (Yao et al., 2009) and with high speed (up to 1000 nucleotides per second).
Is phage DNA single-stranded or double stranded?
What is phage phi29 DNA polymerase?
phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29) (1). This polymerase has exceptional strand displacement and processive synthesis properties (2).
What is Φ29 DNA polymerase used for?
Φ29 DNA polymerase is an enzyme from the bacteriophage Φ29. It is being increasingly used in molecular biology for multiple displacement DNA amplification procedures, and has a number of features that make it particularly suitable for this application. It was discovered and characterised by Spanish scientist Margarita Salas.
Can Phi 29 DNA be synthesized from 32p DNA?
A system that replicates bacteriophage phi 29 DNA with protein p3 covalently attached to the two 5′ ends, using as the only proteins the phi 29 DNA polymerase and the terminal protein, is described. Restriction analysis of the 32P-labeled DNA synthesized in vitro showed that all phi 29 DNA fragments were labeled.
How big is the Bacillus phage φ29 genome?
“Nucleotide sequence of the late region of Bacillus phage Φ29 completes the 19,285-bp sequence of Φ29 genome. Comparison with the homologous sequence of phage PZA”.